anti thy1 1 monoclonal antibody  (Bio-Rad)

Journal: Antioxidants

Article Title: Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy

doi: 10.3390/antiox9010002

Figure Lengend Snippet: Representative pictures of immunohistochemical stained sections for CD44, a marker for MSC, in ten groups of rats show CD44 positive stains in the glomeruli indicated by arrows in MSCs or HMSCs treated kidneys compared to control kidney ( A1–10 ). Anti-Thy1.1 evoked mesangial lysis (indicated by +) and the mesangial matrix accumulation (indicated with *) in the nephritic rats. Intrarenal MSCs transplantation ameliorated anti-Thy1.1-induced nephritis in the rat model. Normoxic (MSC) and hypoxic MSCs (HMSC) markedly reduced the inflammatory cell infiltration in the glomeruli in H&E stains ( B ). The severity of glomerulosclerosis was explored in both normoxic MSC and HMSC treated rats in PAS stains ( C ) and in Masson stains ( D ). Thy1-induced nephritis significantly elevated urinary protein level and the elevated urinary protein level was significantly reduced by intrarenal MSC or HMSC treatment ( E ). Glomerular sclerotic index calculated by the PAS sections among the experimental groups are presented in ( F ). Thy1-induced nephritis significantly increased glomerular sclerosis in all nephritis treated groups as compared to Con group. MSC at cell number (2–5) × 10 5 level and HMSC at cell number (1–5) × 10 5 level significantly reduced sclerosis degree. Glomerular fibrosis determined by the hydroxyproline contents among all groups of animals are presented in ( G ). Thy1-induced nephritis significantly increased renal hydroxyproline content in all nephritis treated groups as compared to Con group. MSC and HMSC significantly reduced renal fibrotic degree. Each graph is amplified at 400×. The scale bar (20 μm) is indicated in each graph. * p < 0.05 vs. Con group. # p < 0.05 vs. Thy1 group. a p < 0.05 vs. Thy1 + 1MSC group. b p < 0.05 vs. Thy1 + 2MSC group. c p < 0.05 vs. Thy1 + 5MSC group.

Article Snippet: Anti-thy1.1 GN was induced by injection of 0.2 mL of phosphate-buffered saline containing 250 μg anti-thy1.1 monoclonal antibody (Cedarlane, Burlington, ON, Canada) into rats via a jugular vein under sodium pentobarbital anesthesia (50 mg/kg, i.p.) at day 0, and 0.2 mL of saline injection into the jugular vein as a control group.

Techniques: Immunohistochemical staining, Staining, Marker, Control, Lysis, Transplantation Assay, Amplification

Journal: Antioxidants

Article Title: Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy

doi: 10.3390/antiox9010002

Figure Lengend Snippet: The oxidative stress index of renal ED-1 ( A ), GRP78 ( B ), Beclin-1 ( C ), LC3-II ( D ), Bax/Bcl-2 ratio ( E ), Caspase-3 ( F ), PARP ( G ) and β-actin ( H ) is determined with western blot among eight groups of rats. The quantitative data showed that anti-Thy1.1 significantly enhanced ED-1, GRP78, Beclin-1, LC3-II, Bax/Bcl-2 ratio, Caspase 3, and PARP in the damaged kidneys. MSCs with three amounts did not significantly reduce these oxidative parameters in the anti-thy1.1 treated kidneys. HMSCs treated anti-thy1.1 kidneys with the number of 5 × 10 5 significantly reduced these parameters as compared to anti-thy1.1 treated group. * p < 0.05 vs. Con group. # p < 0.05 vs. Thy1.1 group.

Article Snippet: Anti-thy1.1 GN was induced by injection of 0.2 mL of phosphate-buffered saline containing 250 μg anti-thy1.1 monoclonal antibody (Cedarlane, Burlington, ON, Canada) into rats via a jugular vein under sodium pentobarbital anesthesia (50 mg/kg, i.p.) at day 0, and 0.2 mL of saline injection into the jugular vein as a control group.

Techniques: Western Blot

Journal: Antioxidants

Article Title: Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy

doi: 10.3390/antiox9010002

Figure Lengend Snippet: Immunohistochemical stains for ED1, GRP78, LC3-II, Caspase1, TUNEL, and Collagen IV in the study groups. Detection of ED1+ cell in the study groups are demonstrated in ( A1–4 ). Semiquantitative assessment revealed infusion of MSCs and showed a reduction in numbers of macrophage/monocyte infiltration (ED1+ cell). HMSCs possessed a better reduction effect ( A5 ). Representative pictures of sections for stress index protein acculumation ( B 1–4), autophagy by LC3-II ( C1–4 ), apoptotic cells by caspase 1( D1–4 ) and TUNEL ( E1–4 ) and collagen deposition ( F1–4 ) in glomeruli. The semiquantitative assessment of these sections revealed anti-Thy1.1 administration increased stress index protein (GRP78) accumulation, autophagy index protein (LC3II) detection, apoptotic (TUNEL, caspase 1) cells, and collagen IV accumulation. MSC infusion ameliorated the increase in GRP78, LC3 II, caspase 1, TUNEL, and collagen IV in kidneys after anti-Thy1.1 infusion. HMSC infusion had greater therapeutic reduction effects. Each graph is amplified at 400×. The scale bar (20 μm) is indicated in the Figure. * p < 0.05 vs. Con group. # p < 0.05 vs. Thy1 group. a p < 0.05 vs. Thy1 + 5MSC group.

Article Snippet: Anti-thy1.1 GN was induced by injection of 0.2 mL of phosphate-buffered saline containing 250 μg anti-thy1.1 monoclonal antibody (Cedarlane, Burlington, ON, Canada) into rats via a jugular vein under sodium pentobarbital anesthesia (50 mg/kg, i.p.) at day 0, and 0.2 mL of saline injection into the jugular vein as a control group.

Techniques: Immunohistochemical staining, TUNEL Assay, Amplification

Journal: Antioxidants

Article Title: Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy

doi: 10.3390/antiox9010002

Figure Lengend Snippet: The index of ROS, ROS enzymatic scavenger expression, master anti-oxidative injury regulator Nrf2 expression, and anti-oxidative response protein expressions among the experimental groups. The accumulation of ROS by 4 HNE stain among the groups are demonstrated from the amplified graph at 400× (scale bar = 20 μm, A1–4 ). The semi-quantitative analyses of these sections revealed that anti-Thy1.1 infusion greatly increased ROS, and HMSCs ameliorated the ROS accumulation ( A5 ). ROS enzymatic scavengers including MnSOD, Cu/ZnSOD and catalase were significantly reduced by antiThy-1 infusion. These enzymes were rescued by HMSCs ( B1–3 ). The nuclear Nrf2 expression is not triggered by anti-Thy 1.1 infusion and is enhanced in the HMSC treated group. Significantly elevated nuclear NFkB expression is noted in anti-Thy1.1 group, which is efficiently suppressed in HMSCs treated group ( C1–3 ). Downstream antioxidative response element protein expression including GCLC, GCLM, GPX are suppressed by anti-Thy 1.1 infusion. These proteins are rescued by HMSC transplantation ( D1–3 ). * p < 0.05 vs. Con group. # p < 0.05 vs. Thy1.1 group.

Article Snippet: Anti-thy1.1 GN was induced by injection of 0.2 mL of phosphate-buffered saline containing 250 μg anti-thy1.1 monoclonal antibody (Cedarlane, Burlington, ON, Canada) into rats via a jugular vein under sodium pentobarbital anesthesia (50 mg/kg, i.p.) at day 0, and 0.2 mL of saline injection into the jugular vein as a control group.

Techniques: Expressing, Staining, Amplification, Transplantation Assay

Journal: eLife

Article Title: Natural Tr1-like cells do not confer long-term tolerogenic memory

doi: 10.7554/eLife.44821

Figure Lengend Snippet:

Article Snippet: Antibody , InVivoMAb mouse monoclonal anti-mouse Thy1.1/CD90.1 (clone 19E12) , BioXcell , Cat: BE0214 , 200 ug/mouse.

Techniques: Flow Cytometry